Caspase 2/p18 Polyclonal antibody

Caspase 2/p18 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24777-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24777-1-AP is Caspase 2/p18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 2/p18 fusion protein Ag20141
Full Name caspase 2, apoptosis-related cysteine peptidase
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight 45 kDa, 32 kDa
GenBank accession numberBC002427
Gene symbol CASP2
Gene ID (NCBI) 835
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Protocols

Product Specific Protocols
WB protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
IHC protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
IF protocol for Caspase 2/p18 antibody 24777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols