Caspase 2/p32/p18 Monoclonal antibody, PBS Only

Caspase 2/p32/p18 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G4C2

Cat no : 66517-1-PBS

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Validation Data Gallery

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  WB analysis of Jurkat using 66517-1-Ig (same clone as 66517-1-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66517-1-Ig (Caspase 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66517-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66517-1-PBS targets Caspase 2/p32/p18 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 2/p32/p18 fusion protein Ag20141
• Full Name: caspase 2, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa, 32 kDa, 18 kDa
• GenBank Accession Number: BC002427
• Gene Symbol: CASP2
• Gene ID (NCBI): 835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.