Caspase 2/p32/p18 Monoclonal antibody

Caspase 2/p32/p18 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G4C2

Cat no : 66517-1-Ig

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Validation Data Gallery

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  WB analysis of Jurkat using 66517-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66517-1-Ig (Caspase 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66517-1-Ig targets Caspase 2/p32/p18 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 2/p32/p18 fusion protein Ag20141
• Full Name: caspase 2, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa, 32 kDa, 18 kDa
• GenBank Accession Number: BC002427
• Gene Symbol: CASP2
• Gene ID (NCBI): 835
• RRID: AB_2881880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).This antibody can recognize the 32 kDa pro-caspase 2 as well as 18 kDa cleaved-caspase 2.