Caspase 3/P17/P19 Recombinant monoclonal antibody

• 

  WB analysis of Jurkat using 82202-1-RR

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 82202-1-RR

  WB result of Caspase 3/P17/P19 antibody (82202-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3/P17/P19 transfected HeLa cells.

• 

  WB analysis using 82202-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 82202-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3/P17/19 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 82202-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3/P17/19 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 82202-1-RR

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3/P17/19 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

Caspase 3/P17/P19 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 82202-1-RR

Clone No.5G20

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CASP3, Caspase3, 5G20, CASP 3, CASP-3

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, HepG2 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 39 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Product Information

82202-1-RR targets Caspase 3/P17/P19 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit, bovine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa, 17 kDa, 19 kDa
• GenBank Accession Number: NM_004346
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• RRID: AB_3086469
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42574
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Acta Pharm Sin B; Design, synthesis, and antitumor activity of novel thioheterocyclic nucleoside derivatives by suppressing the c-MYC pathway; Authors - Xian-Jia Li
mouse	WB	Int Immunopharmacol; CSF1R inhibition agents protect against cisplatin ototoxicity and synergize with immunotherapy for Head and Neck Squamous Cell Carcinoma; Authors - Jiahui Zhang
human	WB	Acta Biochim Biophys Sin (Shanghai); Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression; Authors - Kenan Huang
mouse,human	WB,IHC	Phytomedicine; Cinnamaldehyde alleviates aspirin-induced gastric mucosal injury by regulating pi3k/akt pathway-mediated apoptosis, autophagy and ferroptosis; Authors - Shuguang Yan
mouse	IHC	Biomater Res; Genetically Modified Hepatocytes Targeting Bilirubin and Ammonia Metabolism for the Construction of Bioartificial Liver System; Authors - Ke Wang
mouse	WB	Metabolism; Estradiol contributes to sex differences in resilience to septic-induced metabolic dysregulation and dysfunction in the heart via GPER-1-mediated PPARδ/NLRP3 signaling; Authors - Joseph Adu-Amankwaah