Caspase 4 Monoclonal antibody

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  WB analysis of SGC-7901 using 67398-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67398-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67398-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67398-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67398-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67398-1-Ig (Caspase 4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67398-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67398-1-Ig (Caspase 4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67398-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 67398-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67398-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 4 antibody (67398-1-Ig, Clone: 1D1E12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Caspase 4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 67398-1-Ig

Clone No.1D1E12

Publications(4)

Reviews (2)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CASP4, Caspase4, 1D1E12, CASP 4, CASP-4

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Tested Applications

• Positive WB detected in: SGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

67398-1-Ig targets Caspase 4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 4 fusion protein Ag29488
• Full Name: caspase 4, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43-48 kDa
• GenBank Accession Number: BC017839
• Gene Symbol: Caspase 4
• Gene ID (NCBI): 837
• RRID: AB_2882641
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49662
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Publications

Species	Application	Title
human	IHC	Hum Cell; Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas; Authors - Guiying He
human	WB	Life Sci Alliance; Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzyme; Authors - Amy H Chan
human	WB	ACS Chem Neurosci; Hypoxanthine Produces Rapid Antidepressant Effects by Suppressing Inflammation in Serum and Hippocampus; Authors - Lei Wu
human	WB	Mol Carcinog; Pyroptosis-related gene GSDMC indicates poor prognosis and promotes tumor progression by activating the AKT/mTOR pathway in lung squamous cell carcinoma; Authors - Yi Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dave (Verified Customer) (06-13-2022)

This is a great antibody. Work very efficiently if you use it as the first antibody (worse less well if your membrane has already been deactivated with Peroxide). This product also detect efficiently cleaved Caspase-4 (which can be very challenging with other antibodies)

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human epithelial and myeloid cells

FH

Aldona (Verified Customer) (10-29-2021)

I have no comments

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: THP-1