Caspase 4 Monoclonal antibody

Caspase 4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1E12

Cat no : 67398-1-Ig

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX



Tested Applications

Positive WB detected inSGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67398-1-Ig targets Caspase 4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 4 fusion protein Ag29488
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC017839
Gene Symbol Caspase 4
Gene ID (NCBI) 837
RRIDAB_2882641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4 antibody 67398-1-IgDownload protocol
IHC protocol for Caspase 4 antibody 67398-1-IgDownload protocol
IF protocol for Caspase 4 antibody 67398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Cell

Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas

Authors - Guiying He
humanWB

Life Sci Alliance

Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzyme

Authors - Amy H Chan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Dave (Verified Customer) (06-13-2022)

This is a great antibody. Work very efficiently if you use it as the first antibody (worse less well if your membrane has already been deactivated with Peroxide). This product also detect efficiently cleaved Caspase-4 (which can be very challenging with other antibodies)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human epithelial and myeloid cells
FH

Aldona (Verified Customer) (10-29-2021)

I have no comments

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: THP-1