Caspase 4 Monoclonal antibody
Caspase 4 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1D1E12
Cat no : 67398-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Sample-dependent, check data in validation data gallery |
Product Information
67398-1-Ig targets Caspase 4 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Caspase 4 fusion protein Ag29488 |
Full Name | caspase 4, apoptosis-related cysteine peptidase |
Calculated molecular weight | 377 aa, 43 kDa |
Observed molecular weight | 43-48 kDa |
GenBank accession number | BC017839 |
Gene symbol | CASP4 |
Gene ID (NCBI) | 837 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
IHC protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
IF protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aldona (Verified Customer) (10-29-2021) | I have no comments
|