Caspase 4 Monoclonal antibody

Caspase 4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D1E12

Cat no : 67398-1-Ig

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX



Tested Applications

Positive WB detected inSGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

67398-1-Ig targets Caspase 4 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 4 fusion protein Ag29488
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC017839
Gene symbol CASP4
Gene ID (NCBI) 837
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4 antibody 67398-1-IgDownload protocol
IHC protocol for Caspase 4 antibody 67398-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols