CD2AP Polyclonal antibody

• Featured Product
• KD/KO Validated

CD2AP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 51046-1-AP

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

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Validation Data Gallery

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  WB analysis of mouse kidney using 51046-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 51046-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 51046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 51046-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 51046-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 51046-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51046-1-AP (Cd2ap antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 51046-1-AP

  IP result of anti-Cd2ap (IP:51046-1-AP, 4ug; Detection:51046-1-AP 1:2000) with mouse brain tissue lysate 2560ug.

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  IP experiment of HeLa cells using 51046-1-AP

  IP result of anti-Cd2AP (51046-1-AP for IP and Detection).

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  IF Staining of HepG2 using 51046-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 51046-1-AP

  Immunofluorescent analysis of A431 cells using 51046-1-AP (Cd2ap antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse testis tissue, mouse brain tissue, Jurkat cells, A431 cells, HeLa cells
• Positive IP detected in: mouse brain tissue, HeLa cells
• Positive IF/ICC detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

51046-1-AP targets CD2AP in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD2AP fusion protein Ag0622
• Full Name: CD2-associated protein
• Calculated Molecular Weight: 637 aa, 70 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC157901
• Gene Symbol: Cd2ap
• Gene ID (NCBI): 12488
• RRID: AB_670322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function.; Authors - Sei Yoshida
human	WB	Exp Ther Med; Transforming growth factor-β1-induced podocyte injury is associated with increased microRNA-155 expression, enhanced inflammatory responses and MAPK pathway activation.; Authors - Xintong Zheng
human	WB,IF	J Biol Chem; The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling; Authors - Tomohiko Iwano ; KD Validated
mouse	WB	Int J Mol Sci; The Cleavage-Specific Tau 12A12mAb Exerts an Anti-Amyloidogenic Action by Modulating the Endocytic and Bioenergetic Pathways in Alzheimer's Disease Mouse Model; Authors - Valentina Latina
mouse	WB,IF	J Am Soc Nephrol; Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes; Authors - Naritoshi Shirata
human	WB	Int J Cardiol; Human cytomegalovirus pUL135 protein affects endothelial cell function via CD2AP in Kawasaki disease; Authors - Lili Yu ; KD Validated