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Centrin 1 Monoclonal antibody, PBS Only

Centrin 1 Monoclonal Antibody for IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8D10

Cat no : 68433-1-PBS

Synonyms

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of ARPE-19 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Centrin 1 Monoclonal antibody (68433-1-Ig)

    IF Staining of hTERT-RPE1 using 68433-1-Ig (same clone as 68433-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68433-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68433-1-PBS targets Centrin 1 in IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Centrin 1 fusion protein Ag3529
Full Name centrin, EF-hand protein, 1
Calculated Molecular Weight 172 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC029515
Gene Symbol CETN1
Gene ID (NCBI) 1068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.