Chicken IgY Monoclonal antibody

Chicken IgY Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E12G2

Cat no : 67405-1-Ig

Synonyms

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Validation Data Gallery

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  WB analysis of Recombinant protein using 67405-1-Ig

  100 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  50 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  25 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  12.5 nL chicken egg were subjected to SDS-PAGE at non-reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. .

• 

  WB analysis of Recombinant protein using 67405-1-Ig

  12.5 nL chicken egg were subjected to SDS-PAGE under reducing condition followed by western blot with 67405-1-Ig (chicken IgY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Chicken IgY, Chicken egg

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67405-1-Ig targets Chicken IgY in WB, ELISA applications and shows reactivity with Chicken samples.

• Tested Reactivity: Chicken
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Chicken IgY
• Observed Molecular Weight: 180 kDa, 70 kDa
• RRID: AB_2882646
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).

Publications

Species	Application	Title
		Carbohydr Polym; Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y.; Authors - Hao Li