Chromogranin B Polyclonal antibody

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  WB analysis using 28663-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 28663-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-12 using 28663-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (28663-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Chromogranin B Polyclonal Antibody for WB, FC (Intra), ELISA

Cat No. 28663-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

CHGB, CgB, SCG1, Secretogranin 1, PE-11

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Tested Applications

• Positive WB detected in: PC-12 cells
• Positive FC (Intra) detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28663-1-AP targets Chromogranin B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29920

  Product name: Recombinant human CHGB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 21-328 aa of BC000375

  Sequence: MPVDNRNHNEGMVTRCIIEVLSNALSKSSAPPITPECRQVLKTSRKDVKDKETTENENTKFEVRLLRDPADASEAHESSSRGEAGAPGEEDIQGPTKADTEKWAEGGGHSRERADEPQWSLYPSDSQVSEEVKTRHSEKSQREDEEEEEGENYQKGERGEDSSEEKHLEEPGETQNAFLNERKQASAIKKEELVARSETHAAGHSQEKTHSREKSSQESGEEAGSQENHPQESKGQPRSQEESEEGEEDATSEVDKRRTRPRHHHGRSRPDRSSQGGSLPSEEKGHPQEESEESNVSMASLGEKRDHH

• Full Name: chromogranin B (secretogranin 1)
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC000375
• Gene Symbol: Chromogranin B
• Gene ID (NCBI): 1114
• RRID: AB_2881188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05060
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.