Claudin 16 Recombinant monoclonal antibody

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• Claudin 16 Recombinant monoclonal antibody (82954-2-RR)

• 

  WB analysis using 82954-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82954-2-RR (Claudin 16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82954-2-RR

  WB result of Claudin 16 antibody (82954-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 16 transfected HepG2 cells.

• Featured Product
• KD/KO Validated

Claudin 16 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82954-2-RR

Clone No.251611H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Claudin-16, CLDN16, Paracellin-1, PCLN1, PCLN-1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, ACHN cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82954-2-RR targets Claudin 16 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: claudin 16
• Calculated Molecular Weight: 305 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC069682
• Gene Symbol: CLDN16
• Gene ID (NCBI): 10686
• RRID: AB_3743446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y5I7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They have similar structures with four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-16 is primarily found in the kidneys and is responsible for regulating the reabsorption of magnesium and calcium ions. Mutations in the Claudin-16 gene can lead to a rare genetic disorder called familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC).