Claudin 3 Recombinant monoclonal antibody

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• Claudin 3 Recombinant monoclonal antibody (86697-1-RR)

• 

  WB analysis using 86697-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86697-1-RR (Claudin 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86697-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86697-1-RR (Claudin 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 86697-1-RR

  IP result of anti-Claudin 3 (IP:86697-1-RR, 4ug; Detection:86697-1-RR 1:1000) with HT-29 cells lysate 1760 ug.

Claudin 3 Uni-rAb^® Recombinant Antibody for WB, IP, ELISA

Cat No. 86697-1-RR

Clone No.251540A11

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CLDN3, Claudin3, Claudin-3, CPE R 2, CPE R2

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Tested Applications

• Positive WB detected in: HT-29 cells, mouse colon tissue, Calu-3 cells, MCF-7 cells, rat colon tissue
• Positive IP detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

86697-1-RR targets Claudin 3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: claudin 3
• Calculated Molecular Weight: 220 aa, 23 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC016056
• Gene Symbol: Claudin 3
• Gene ID (NCBI): 1365
• RRID: AB_3745061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O15551
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin-3 (CLDN3) is one of 27 known members of the claudin family. Together with occludin and other junctional adhesion molecules, the claudins form the tight-junctions (TJs) that regulate intercellular permeability. Altered CLDN3 expression has been linked to tumor progression in multiple tumor types.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Food Res Int; Fermented grape pomace alleviates Salmonella typhimurium-induced Hepatointestinal inflammation: FXR mediates protection by orchestrating bile acid homeostasis and suppressing the colonic NLRP3 Inflammasome.; Authors - Jing Zhang