Claudin 4 Recombinant monoclonal antibody, PBS Only

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• Claudin 4 Recombinant monoclonal antibody, PBS Only (86433-8-PBS)

• 

  WB analysis using 86433-8-RR (same clone as 86433-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86433-8-RR (Claudin 4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86433-8-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86433-8-RR (same clone as 86433-8-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86433-8-RR (Claudin 4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86433-8-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 86433-8-RR (same clone as 86433-8-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86433-8-RR (Claudin 4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86433-8-PBS in a different storage buffer formulation.

Claudin 4 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86433-8-PBS

Clone No.251202E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CLDN4, CPE R, CPE receptor, CPER, CPE-R

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Product Information

86433-8-PBS targets Claudin 4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: claudin 4
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC000671
• Gene Symbol: Claudin 4
• Gene ID (NCBI): 1364
• RRID: AB_3744879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14493
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with similar structures. They have four transmembrane domains, with the N- and C-termini in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624). Claudin 4 is the receptor for B. fragilis toxin (BFT), an important oncogenic protein. Blocking the interaction between BFT and Claudin-4 can inhibit the cleavage of the substrate E-cadherin by BFT and reverse the tissue damage induced by BFT in mice (PMID: 42020735).