Claudin 4 Recombinant monoclonal antibody

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• Claudin 4 Recombinant monoclonal antibody (86433-8-RR)

• 

  WB analysis using 86433-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86433-8-RR (Claudin 4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86433-8-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86433-8-RR (Claudin 4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 86433-8-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86433-8-RR (Claudin 4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Claudin 4 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86433-8-RR

Clone No.251202E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CLDN4, CPE R, CPE receptor, CPER, CPE-R

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, human placenta tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86433-8-RR targets Claudin 4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: claudin 4
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC000671
• Gene Symbol: Claudin 4
• Gene ID (NCBI): 1364
• RRID: AB_3744879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14493
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with similar structures. They have four transmembrane domains, with the N- and C-termini in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624). Claudin 4 is the receptor for B. fragilis toxin (BFT), an important oncogenic protein. Blocking the interaction between BFT and Claudin-4 can inhibit the cleavage of the substrate E-cadherin by BFT and reverse the tissue damage induced by BFT in mice (PMID: 42020735).