Cleaved Caspase 4/P20 Recombinant monoclonal antibody

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• Cleaved Caspase 4/P20 Recombinant monoclonal antibody (85770-1-RR)

Cleaved Caspase 4/P20 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85770-1-RR Copied!

Clone No. 243150D6

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CASP4, Caspase4, CASP 4, CASP-4, Caspase 4

• 

  WB analysis using 85770-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85770-1-RR (Cleaved Caspase 4/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 85770-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85770-1-RR (Cleaved Caspase 4/P20 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85770-1-RR targets Cleaved Caspase 4/P20 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: caspase 4, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC017839
• Gene Symbol: Caspase 4
• Gene ID (NCBI): 837
• RRID: AB_3744362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49662
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase-4 participates in cleavage and activates gasdermin D (GSDMD), leading to cell membrane perforation, cell swelling and rupture, thereby inducing pyroptosis.

Publications

What published studies show

The Cleaved Caspase 4/P20 Recombinant monoclonal antibody (243150D6), catalog number 85770-1-RR, has 1 total citation. The citation appears in Cell Reports (2026), a study investigating how the pyroptosis cascade between inflammatory endothelial cells and microglia facilitates blood-brain barrier disruption in bacterial meningitis. The associated research fields include pyroptosis, neuroinflammation, and infectious disease pathogenesis.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cell Rep; The pyroptosis cascade between inflammatory endothelial cells and microglia facilitates BBB disruption in bacterial meningitis.; Authors - Yangyang Zheng