Collagen Type I Recombinant antibody, PBS Only

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  WB analysis of SK-N-SH using 86093-1-RR (same clone as 86093-1-PBS)

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 86093-1-RR (COL1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86093-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86093-1-RR (same clone as 86093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86093-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 86093-1-RR (same clone as 86093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86093-1-PBS in a different storage buffer formulation.

Collagen Type I Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 86093-1-PBS

Clone No.250534B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

COL1A1, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I, col1

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Product Information

86093-1-PBS targets Collagen Type I in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Collagen Type I fusion protein Ag27389
• Full Name: collagen, type I, alpha 1
• Calculated Molecular Weight: 1464 aa, 139 kDa
• Observed Molecular Weight: 140 kDa, 200kDa
• GenBank Accession Number: BC036531
• Gene Symbol: COL1A1
• Gene ID (NCBI): 1277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02452
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).