Collagen Type I Recombinant antibody, PBS Only

Collagen Type I Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 86093-1-PBS
Clone No.250534B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

COL1A1, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I, col1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

86093-1-PBS targets Collagen Type I in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Collagen Type I fusion protein Ag27389 Predict reactive species
Full Name collagen, type I, alpha 1
Calculated Molecular Weight 1464 aa, 139 kDa
Observed Molecular Weight140 kDa, 200kDa
GenBank Accession NumberBC036531
Gene Symbol COL1A1
Gene ID (NCBI) 1277
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02452
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).

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