Tested Applications
| Positive WB detected in | SK-N-SH cells |
| Positive IHC detected in | human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86093-1-RR targets Collagen Type I in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag27389 Product name: Recombinant human COL1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 72-141 aa of BC036531 Sequence: DVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLP Predict reactive species |
| Full Name | collagen, type I, alpha 1 |
| Calculated Molecular Weight | 1464 aa, 139 kDa |
| Observed Molecular Weight | 140 kDa, 200kDa |
| GenBank Accession Number | BC036531 |
| Gene Symbol | COL1A1 |
| Gene ID (NCBI) | 1277 |
| RRID | AB_3744614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Collagen Type I antibody 86093-1-RR | Download protocol |
| IHC protocol for Collagen Type I antibody 86093-1-RR | Download protocol |
| WB protocol for Collagen Type I antibody 86093-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Collagen Type I Recombinant monoclonal antibody (250534B1), catalog number 86093-1-RR, has 14 citations published in journals including Bioactive Materials, Advanced Science, Advanced Healthcare Materials, Materials Today Bio, International Journal of Biological Macromolecules, ACS Applied Materials & Interfaces, Frontiers in Pharmacology, Scientific Reports, Journal of Biological Chemistry, and Cell Biochemistry and Biophysics. Research fields span bone regeneration, osteosarcoma therapy, ferroptosis, wound healing, osteoporosis, liver cirrhosis, colorectal cancer, and fibrosis.
| Species | Application | Title |
|---|---|---|
Bioact Mater NIR-II-activated supramolecular LDH nanoarchitecture integrated with 3D-printed bioactive glass for hypoxia-resistant photodynamic osteosarcoma therapy and bone regeneration. | ||
Adv Sci (Weinh) Ubiquitination of ACSL4 by Parkin Suppresses Ferroptosis and Rescues Glucocorticoid-Induced Bone Loss | ||
Adv Healthc Mater Ascorbic Acid Modulates Collagen Properties in Glucocorticoid-Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation | ||
Mater Today Bio Graphene oxide coating on two-dimensional glass substrates and three-dimensional zirconia scaffolds for bone tissue regeneration. | ||
Int J Biol Macromol Injectable oxidized sodium alginate/gelatin (OSA/Gel) hydrogel loaded with MgO/SZS for ion-mediated osteogenesis and macrophage polarization. |
