Collagen Type I Recombinant monoclonal antibody

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• Collagen Type I Recombinant monoclonal antibody (86093-1-RR)

• 

  WB analysis of SK-N-SH using 86093-1-RR

  SK-N-SH cells were subjected to SDS PAGE followed by western blot with 86093-1-RR (COL1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 86093-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86093-1-RR (COL1A1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HSC-T6 using 86093-1-RR

  1x10^6 HSC-T6 cells were intracellularly stained with 0.25 ug Collagen Type I Recombinant monoclonal antibody (86093-1-RR, Clone:250534B1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

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  Affinity and Kinetic Characterization of 86093-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86093-1-RR against Human Collagen Type I were performed. The affinity constant is 4.37 nM.

Collagen Type I Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 86093-1-RR

Clone No.250534B1

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

COL1A1, Alpha 1 type I collagen, Alpha-1 type I collagen, COL I, col1

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Tested Applications

• Positive WB detected in: SK-N-SH cells
• Positive IHC detected in: human colon tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

86093-1-RR targets Collagen Type I in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27389

  Product name: Recombinant human COL1A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 72-141 aa of BC036531

  Sequence: DVICDETKNCPGAEVPEGECCPVCPDGSESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLP

• Full Name: collagen, type I, alpha 1
• Calculated Molecular Weight: 1464 aa, 139 kDa
• Observed Molecular Weight: 140 kDa, 200kDa
• GenBank Accession Number: BC036531
• Gene Symbol: COL1A1
• Gene ID (NCBI): 1277
• RRID: AB_3744614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02452
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Mutations in COL1A1 are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. This antibody raised against a synthesized peptide corresponding to 1206-1218 aa of human pro-alpha 1 chain of type I collagen recognize collagen alpha-1(I) chain. The presence of unprocessed, intermediate, and mature chains of type I collagen was clearly detected only in static constructs. Indeed, in sponges cultured under perfusion the presence of type I collagen was mainly restricted to mature chains, suggesting that HACs were no longer actively producing type I collagen (PMID: 27584727). It is reported that the molecular weight is 200kDa(PMID: 28526934).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Front Pharmacol; Exploring the mechanism of action of Jinqiancao granules against liver cirrhosis based on the liver-gut axis.; Authors - Fan-E. Cheng
human	WB	Adv Sci (Weinh); Promotion of DFU Wound Healing via BRG1-COL16A1 Axis in Fibroblasts.; Authors - Penghui Wang
		Adv Healthc Mater; Ascorbic Acid Modulates Collagen Properties in Glucocorticoid-Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation; Authors - Micaila DE Curtis
human	WB	Mater Today Bio; Graphene oxide coating on two-dimensional glass substrates and three-dimensional zirconia scaffolds for bone tissue regeneration.; Authors - Ke Liu
rat	WB	Sci Rep; Development of a novel external fixation device for inducing knee arthrofibrosis in rats.; Authors - Jiameng Jia