Collagen Type II Recombinant monoclonal antibody

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• Collagen Type II Recombinant monoclonal antibody (86139-2-RR)

Collagen Type II Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 86139-2-RR Copied!

Clone No. 250506B7

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

• 

  WB analysis using 86139-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86139-2-RR (Collagen Type II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of TT using 86139-2-RR

  TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 86139-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 86139-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human lung cancer using 86139-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Collagen Type II antibody (86139-2-RR, Clone: 250506B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human lung cancer using 86139-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Collagen Type II antibody (86139-2-RR, Clone: 250506B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 86139-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86139-2-RR against Human Collagen Type II were performed. The affinity constant is 4.96 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: TT cells, ATDC-5 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86139-2-RR targets Collagen Type II in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag29265

  Product name: Recombinant human COL2A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-63 aa of BC007252

  Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART

• Full Name: collagen, type II, alpha 1
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC007252
• Gene Symbol: COL2A1
• Gene ID (NCBI): 1280
• RRID: AB_3744654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02458
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.

Publications

What published studies show

Collagen Type II Recombinant monoclonal antibody (250506B7), catalog number 86139-2-RR, has 16 citations. These appear in journals including Bioactive Materials, Carbohydrate Polymers, Advanced Healthcare Materials, Journal of Ethnopharmacology, Life Sciences, Bone & Joint Research, Biological Direct, Chemical Biology & Interaction, Journal of Orthopaedic Surgery and Research, Tissue & Cell, Acta Histochemica, Journal of Molecular Histology, Parasite Immunology, Cell Biochemistry and Biophysics, and Experimental Molecular Medicine. Research fields center on osteoarthritis, cartilage degeneration, intervertebral disc degeneration, rheumatoid arthritis, chondrocyte apoptosis/pyroptosis, and macrophage polarization.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Bioact Mater; A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential.; Authors - Liwen Zheng
rat	WB, IHC, IF	Carbohydr Polym; Ultrasound-triggered injectable piezoelectric nanocomposite hyaluronic acid-based hydrogel for modulating inflammation and chondrogenesis in osteoarthritis treatment.; Authors - Juntao Lin
mouse	IF, IHC	Adv Healthc Mater; Remodeling Cellular Uptake via Inducing Clustering of Urokinase Receptors in Hydrogel Microspheres for Alleviating Cartilage Senescence.; Authors - Zhiqi Lin
mouse	WB,IF	J Ethnopharmacol; Integrated prioritization of candidate quality markers for Smilacis Chinae Rhizoma under the five-principle framework through chemical profiling, effectiveness evaluation, and bionic sensing.; Authors - Huiyin Xia
mouse	IHC	Life Sci; Compressive stress induces cartilage endplate degeneration through the Piezo1/YAP-TEAD/NLRP3 axis.; Authors - Tong Cao ; KO Validated
		Biol Direct; Programmed cell death-related gene S100A9 promotes macrophage M1 polarization and chondrocyte apoptosis in rheumatoid arthritis.; Authors - Qingyuan Xu