Collagen Type II Recombinant monoclonal antibody

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  WB analysis of TT using 86139-2-RR

  TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 86139-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 86139-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86139-2-RR (Collagen Type II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 86139-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86139-2-RR against Human Collagen Type II were performed. The affinity constant is 4.96 nM.

Collagen Type II Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86139-2-RR

Clone No.250506B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

COL2, COL2A1, Alpha 1 type II collagen, Alpha-1 type II collagen, ANFH

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Tested Applications

• Positive WB detected in: TT cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86139-2-RR targets Collagen Type II in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag29265

  Product name: Recombinant human COL2A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-63 aa of BC007252

  Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART

• Full Name: collagen, type II, alpha 1
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC007252
• Gene Symbol: COL2A1
• Gene ID (NCBI): 1280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02458
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.