Tested Applications
| Positive WB detected in | TT cells, ATDC-5 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 5 publications below |
| IF | See 4 publications below |
Product Information
86139-2-RR targets Collagen Type II in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag29265 Product name: Recombinant human COL2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC007252 Sequence: MSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPART Predict reactive species |
| Full Name | collagen, type II, alpha 1 |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC007252 |
| Gene Symbol | COL2A1 |
| Gene ID (NCBI) | 1280 |
| RRID | AB_3744654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02458 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Collagen Type II antibody 86139-2-RR | Download protocol |
| WB protocol for Collagen Type II antibody 86139-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Histochem Stiffness-tunable alginate-hyaluronic acid-collagen hydrogel mimics chondrocyte microenvironment for osteoarthritis pathophysiological modeling. | ||
J Orthop Surg Res SENP1 mitigates intervertebral disc degeneration: mechanisms involving suppression of pyroptosis and apoptosis in nucleus pulposus cells. | ||
J Mol Histol Activated Rac1 promotes chondrocyte apoptosis through the NF-κB pathway in lumbar facet joint osteoarthritis. | ||
Parasite Immunol Schistosoma japonicum-Derived Peptide SJMHE1 Attenuates Osteoarthritis by Promoting Synovial M2 Macrophage Polarisation. | ||
Biol Direct Programmed cell death-related gene S100A9 promotes macrophage M1 polarization and chondrocyte apoptosis in rheumatoid arthritis. | ||
Carbohydr Polym Ultrasound-triggered injectable piezoelectric nanocomposite hyaluronic acid-based hydrogel for modulating inflammation and chondrogenesis in osteoarthritis treatment. |
