TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The COL2A1 gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found primarily in cartilage and the vitreous humor of the eye. Type II collagen is essential for the formation of the extracellular matrix in cartilage and other connective tissues, providing structure and strength to these tissues.
Protocols
Product Specific Protocols
WB protocol for Collagen Type II antibody 86139-2-RR
TT cells were subjected to SDS PAGE followed by western blot with 86139-2-RR (COL2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Collagen Type II Recombinant antibody
Catalog Number
86139-2-RR
Citations
-
Dilutions
WB : 1:2000-1:10000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
