COL5A1 Monoclonal antibody

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• COL5A1 Monoclonal antibody (67604-1-Ig)

COL5A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67604-1-Ig Copied!

Clone No. 2C2C2

Publications (17)

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2C2C2, Collagen alpha 1(V) chain, Collagen alpha-1(V) chain, Collagen Type V

• 

  WB analysis of HT-1080 using 67604-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 67604-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse colon using 67604-1-Ig

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67604-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 67604-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 67604-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Collagen Type V antibody (67604-1-Ig, Clone: 2C2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, mouse colon tissue, pig colon tissue, rat colon tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67604-1-Ig targets COL5A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  native protein

• Full Name: collagen, type V, alpha 1
• Calculated Molecular Weight: 184 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: NM_000093
• Gene Symbol: COL5A1
• Gene ID (NCBI): 1289
• RRID: AB_2882810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P20908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-COL5A1 (Cat# 67604-1-Ig) has 17 citations across journals including Mol Cancer, J Adv Res, Theranostics, Nat Cardiovasc Res, Cell Death Dis, Stem Cell Res Ther, Cell Signal, and others. Research fields span oncology (thyroid, lung, renal, melanoma, pancreatic, ovarian, glioblastoma), fibrosis (intestinal, kidney), cardiovascular disease (aortic dissection, atherosclerosis), osteoarthritis, gouty arthritis, reproductive biology, and collagen extracellular matrix research. Applications include WB, IHC, and IF in human, mouse, and rat samples.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Mol Cancer; CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment; Authors - Zongfu Pan
human	WB	J Adv Res; ALKBH5 modulates COL5A1 mRNA stability via m6A demethylation to promote tobacco carcinogen NNK-induced lung cancer occurrence and progression.; Authors - Yiheng Du ; KD Validated
		J Adv Res; Mesenteric adipose-derived exosomal TINAGL1 enhances intestinal fibrosis in Crohn's Disease via SMAD4; Authors - Yidong Chen
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	IF	Nat Cardiovasc Res; Single-cell spatial transcriptomics of tertiary lymphoid organ-like structures in human atherosclerotic plaques; Authors - Zhichao Lai
human	WB,IHC	Cell Death Dis; CircIQGAP1-CARM1 axis promotes renal cell carcinoma progression through glycolytic reprogramming.; Authors - Ruyue Jia