Collagen Type V Monoclonal antibody, PBS Only

Collagen Type V Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2C2

Cat no : 67604-1-PBS

Synonyms

COL5A1, Collagen alpha 1(V) chain, Collagen Type V, collagen, type V, alpha 1

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Validation Data Gallery

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  WB analysis of HT-1080 using 67604-1-Ig (same clone as 67604-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67604-1-Ig (same clone as 67604-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67604-1-Ig (same clone as 67604-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse colon using 67604-1-Ig (same clone as 67604-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 67604-1-Ig (Collagen Type V antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67604-1-Ig (same clone as 67604-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67604-1-Ig (Collagen Type V antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67604-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67604-1-PBS targets Collagen Type V in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified native protein corresponding to human collagen type V
• Full Name: collagen, type V, alpha 1
• Calculated Molecular Weight: 184 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: NM_000093
• Gene Symbol: COL5A1
• Gene ID (NCBI): 1289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.