CoraLite®594-conjugated Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H10B1

Cat no : CL594-66824

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI , collagen, type VI, alpha 1, OPLL

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-66824

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66824

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL594-66824

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Collagen Type VI antibody (CL594-66824, Clone: 3H10B1 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human liver cancer tissue, human colon cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66824 targets Collagen Type VI in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Collagen Type VI fusion protein Ag11809
• Full Name: collagen, type VI, alpha 1
• Calculated Molecular Weight: 1028 aa, 109 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC052575
• Gene Symbol: COL6A1
• Gene ID (NCBI): 1291
• RRID: AB_2920045
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.