CoraLite®594-conjugated Collagen Type VI Monoclonal antibody

Collagen Type VI Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H10B1

Cat no : CL594-66824

Synonyms

COL6A1, Collagen alpha 1(VI) chain, Collagen Type VI , collagen, type VI, alpha 1, OPLL



Tested Applications

Positive IF detected inhuman liver cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 66824-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66824 targets Collagen Type VI in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag11809
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC052575
Gene symbol COL6A1
Gene ID (NCBI) 1291
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
IF protocol for CL594 Collagen Type VI antibody CL594-66824Download protocol
Standard Protocols
Click here to view our Standard Protocols