Collagen Type XXVIII Polyclonal antibody

Collagen Type XXVIII Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 24401-1-AP

Synonyms

COL28, COL28A1, Collagen alpha 1(XXVIII) chain, Collagen Type XXVIII, collagen, type XXVIII, alpha 1

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Validation Data Gallery

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  WB analysis using 24401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24401-1-AP (Collagen Type XXVIII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 24401-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 24401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Collagen Type XXVIII antibody (24401-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF detected in: A431 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24401-1-AP targets Collagen Type XXVIII in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type XXVIII fusion protein Ag21459
• Full Name: collagen, type XXVIII, alpha 1
• Calculated Molecular Weight: 1125 aa, 117 kDa
• Observed Molecular Weight: 72 kDa, 100-117 kDa
• GenBank Accession Number: BC136892
• Gene Symbol: COL28A1
• Gene ID (NCBI): 340267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL28A1 (collagen type XXVIII alpha 1 chain), also known as COL28. It is expected to be located in the basement membranes, cytoplasm and nucleus. And the protein is mainly expressed in salivary gland and adrenal. This antibody can recognize the 117 kDa and 72 kDa isoforms of target. COL28A1 is a new collagen that cannot clearly be assigned to any collagen subgroup, despite the fact that it contains VWA domains. Ongoing investigations will shed further light on the role of COL28A1 in the assembly of nerve fiber basement membranes. Although no human mutation has been associated with COL28A1, it is tempting to speculate that mutations in this gene would lead to neurodegenerative disease (PMID: 16330543).