Complement C6 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA sample test of MP02310-2

  Urine of six individual healthy human donors was measured. The Complement C6 concentration of detected samples was determined to be 443.1 pg/mL with a range of 54.8-1,501.2 pg/mL.

• 

  Sandwich ELISA sample test of MP02310-2

  Human milk of six individual healthy female donors was measured. The Complement C6 concentration of detected samples was determined to be 139.3 ng/mL with a range of 61.9-278.6 ng/mL.

• 

  Sandwich ELISA sample test of MP02310-2

  Saliva of six individual healthy female donors was measured. The Complement C6 concentration of detected samples was determined to be 4.6 ng/mL with a range of 1.9-13.8 ng/mL.

• 

  Sandwich ELISA sample test of MP02310-2

  Serum of six individual healthy human donors was measured. The Complement C6 concentration of detected samples was determined to be 21,824.0 ng/mL with a range of 676.5-56,025.2 ng/mL.

• 

  Sandwich ELISA standard curve of MP02310-2

  Sandwich ELISA standard curve of MP02310-2, Human Complement C6 Recombinant Matched Antibody Pair - PBS only. 86286-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4529. 86286-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Cytometric bead array sample test of MP02310-1

  The mean Complement C6 concentration was determined to be 17.3 ug/mL in human serum.

• 

  Cytometric bead array standard curve of MP02310-1

  Cytometric bead array standard curve of MP02310-1, Complement C6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86286-2-PBS. Detection antibody: 86286-1-PBS. Standard: Eg4529. Range: 0.313-40 ng/mL.

• 

  Affinity of 86286-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 86286-2-PBS against Human Complement C6 was performed. The affinity constant is 2.26 nM.

Complement C6 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 86286-2-PBS

Clone No.250971H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

C6, complement component 6, Complement component C6

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Product Information

86286-2-PBS targets Complement C6 as part of a matched antibody pair:

MP02310-1: 86286-2-PBS capture and 86286-1-PBS detection (validated in Cytometric bead array)

MP02310-2: 86286-2-PBS capture and 86286-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4529

  Product name: Recombinant Human Complement C6 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-934 aa of BC035723

  Sequence: CFCDHYAWTQWTSCSKTCNSGTQSRHRQIVVDKYYQENFCEQICSKQETRECNWQRCPINCLLGDFGPWSDCDPCIEKQSKVRSVLRPSQFGGQPCTAPLVAFQPCIPSKLCKIEEADCKNKFRCDSGRCIARKLECNGENDCGDNSDERDCGRTKAVCTRKYNPIPSVQLMGNGFHFLAGEPRGEVLDNSFTGGICKTVKSSRTSNPYRVPANLENVGFEVQTAEDDLKTDFYKDLTSLGHNENQQGSFSSQGGSSFSVPIFYSSKRSENINHNSAFKQAIQASHKKDSSFIRIHKVMKVLNFTTKAKDLHLSDVFLKALNHLPLEYNSALYSRIFDDFGTHYFTSGSLGGVYDLLYQFSSEELKNSGLTEEEAKHCVRIETKKRVLFAKKTKVEHRCTTNKLSEKHEGSFIQGAEKSISLIRGGRSEYGAALAWEKGSSGLEEKTFSEWLESVKENPAVIDFELAPIVDLVRNIPCAVTKRNNLRKALQEYAAKFDPCQCAPCPNNGRPTLSGTECLCVCQSGTYGENCEKQSPDYKSNAVDGQWGCWSSWSTCDATYKRSRTRECNNPAPQRGGKRCEGEKRQEEDCTFSIMENNGQPCINDDEEMKEVDLPEIEADSGCPQPVPPENGFIRNEKQLYLVGEDVEISCLTGFETVGYQYFRCLPDGTWRQGDVECQRTECIKPVVQEVLTITPFQRLYRIGESIELTCPKGFVVAGPSRYTCQGNSWTPPISNSLTCEKDTLTKLKGHCQLGQKQSGSECICMSPEEDCSHHSEDLCVFDTDSNDYFTSPACKFLAEKCLNNQQLHFLHIGSCQDGRQLEWGLERTRLSSNSTKKESCGYDTCYDWEKCSASTSKCVCLLPPQCFKGGNQLYCVKMGSSTSEKTLNICEVGTIRCANRKMEILHPGKCLA

• Full Name: complement component 6
• Calculated Molecular Weight: 934 aa, 105 kDa
• GenBank Accession Number: BC035723
• Gene Symbol: C6
• Gene ID (NCBI): 729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13671
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.