Conjugation CleanAb Kit for Mouse IgG, Magnetic Agarose

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• Conjugation CleanAb Kit for Mouse IgG, Magnetic Agarose (CK004)

Validation Data Gallery

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  Mouse IgG1 (anti Lamin B1; 66095-1-Ig) was conjugated with NHS-Azide (lane 1) and then with DBCO-Oligo (15nt) (lane 2). Post-conjugation clean-up was done using CleanAb Kit (magnetic agarose) for rabbit IgG (lanes 3-5). Excess oligo was successfully removed as shown in SYBR Gold DNA staining and IgG eluted with high recovery (~70%) at neutral pH (lane 4). Desalting was done with Zeba spin desalting columns (lane 5). No IgG remains on beads after elution (lane 6).

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  Mouse IgG1 (anti Lamin B1; 66095-1-Ig, lane 1) was bound to CleanAb rabbit beads (magnetic agarose) (flowthrough in lane 2), then functionalized on bead with NHS-Azide (flowthrough in lane 3) and then with DBCO-Oligo (15nt) (flowthrough in lane 4, visible in SYBR Gold staining). Excess oligo was successfully removed as shown in SYBR Gold DNA staining and the oligo-conjugated IgG was eluted with high recovery at neutral pH (lane 6).

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  Mouse IgG1 (anti Lamin B1; 66095-1-Ig) was conjugated with NHS-dyes (lane 1 respectively). Post-conjugation clean-up was done using CleanAb Kit (magnetic agarose) for Rabbit IgG (lanes 2-4). At least 95% of excess dye was successfully removed as evident from the faint excess dye band (lane 4). The conjugated IgG was eluted with high recovery (>70%) at neutral pH (lane 4). Desalting was done with Zeba spin desalting columns to exchange buffer after elution (lane 6), successfully removing all residual dye (buffer exchange is only recommended for long term storage or if required for downstream assays). No IgG remains on beads (lane 7). Note: the CleanAb Elution Buffer can interfere with SDS gels, which can make the CleanAb elution appear smeary.

Conjugation CleanAb Kits for Mouse IgG are designed for fast & easy removal of excess conjugate (e.g. oligos, small molecule fluorescent dyes) from conjugated mouse antibodies post conjugation or after on-bead conjugation. The Kit uses agarose (CK003) or magnetic agarose beads (CK004) coated with Fab binding proteins, optimized for elution at native pH (pH 7.5), facilitating gentle recovery of your sample from the beads, with high efficiency and excellent recovery rates.

Cat No. CK004

Reactivity

Mouse IgG

Applications

Purification of conjugated IgG

Conjugate

Magnetic Agarose

Synonyms

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Product Information

Conjugation CleanAb Kits for Mouse IgG are designed for fast & easy removal of excess conjugate (e.g. oligos, small molecule fluorescent dyes) from conjugated mouse antibodies post conjugation or after on-bead conjugation. The Kit uses agarose (CK003) or magnetic agarose beads (CK004) coated with Fab binding proteins, optimized for elution at native pH (pH 7.5), facilitating gentle recovery of your sample from the beads, with high efficiency and excellent recovery rates.

• Description: Conjugation CleanAb Kits for Mouse IgG are designed for fast & easy removal of excess conjugate (e.g. oligos, small molecule fluorescent dyes) from conjugated mouse antibodies post conjugation or after on-bead conjugation. The Kit uses agarose (CK003) or magnetic agarose beads (CK004) coated with Fab binding proteins, optimized for elution at native pH (pH 7.5), facilitating gentle recovery of your sample from the beads, with high efficiency and excellent recovery rates.;

  • High capacity & high specificity Beads --> Minimized sample loss;

  • Fast & efficient removal of excess DNA oligo --> No oligo-derived background in end-application;

  • Elution at native pH 7.5 --> Preserves your conjugate;

  • High recovery rate of 60 – 80% --> Saves your sample and money

• Applications: Purification
• Binding capacity: > 6 mg IgG per 1 ml bead slurry
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• CleanAb Kit Wash Buffer: 10 mM Tris/Cl pH 7.5, 150 mM NaCl, 0.05 % Nonidet™ P40 Substitute, 0.5 mM EDTA
• Bead dilution: /
• Class: Recombinant - Animal free production
• Immunogen: Mouse IgG
• RRID: /
• Storage Buffer: 20% Ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!
• Shipping: Ambient Temp
• Size: 1 ml

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