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Cyclin D3 Polyclonal antibody

Cyclin D3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26755-1-AP

Synonyms

CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3



Tested Applications

Positive WB detected inC6 cells, K-562 cells, Jurkat cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26755-1-AP is Cyclin D3 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cyclin D3 fusion protein Ag25151
Full Name cyclin D3
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC011616
Gene symbol CCND3
Gene ID (NCBI) 896
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

Protocols

Product Specific Protocols
WB protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IHC protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IF protocol for Cyclin D3 antibody 26755-1-APDownload protocol
IP protocol for Cyclin D3 antibody 26755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Identification of a Proteomic Signature of Senescence in Primary Human Mammary Epithelial Cells

Authors - Alireza Delfarah
humanWB

Transl Lung Cancer Res

Deubiquitinase USP5 promotes non-small cell lung cancer cell proliferation by stabilizing cyclin D1.

Authors - Zhiwei Zhang
humanIHC

Cancer Gene Ther

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Authors - Minhai Dong
mouseWB

J Cell Mol Med

Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21Cip1 and p27Kip1.

Authors - Dan Zhang
humanWB

Mol Ther Oncolytics

S119N Mutation of the E3 Ubiquitin Ligase SPOP Suppresses SLC7A1 Degradation to Regulate Hepatoblastoma Progression.

Authors - Weijing He
mouseWB

Mol Cell Endocrinol

ActivinA activates Notch1-Shh signaling to regulate proliferation in C2C12 skeletal muscle cells.

Authors - Li Ma