|Positive WB detected in||C6 cells, K-562 cells, Jurkat cells, HeLa cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26755-1-AP is Cyclin D3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cyclin D3 fusion protein Ag25151|
|Full Name||cyclin D3|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC011616|
|Gene ID (NCBI)||896|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.
|Product Specific Protocols|
|WB protocol for Cyclin D3 antibody 26755-1-AP||Download protocol|
|IHC protocol for Cyclin D3 antibody 26755-1-AP||Download protocol|
|IF protocol for Cyclin D3 antibody 26755-1-AP||Download protocol|
|IP protocol for Cyclin D3 antibody 26755-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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