Cyclophilin E Recombinant antibody

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  WB analysis using 85952-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85952-1-RR (Cyclophilin E antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85952-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85952-1-RR against Human Cyclophilin E were performed. The affinity constant is 58.2 nM.

Cyclophilin E Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85952-1-RR

Clone No.250296A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, Jurkat cells, BxPC-3 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85952-1-RR targets Cyclophilin E in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclophilin E fusion protein Ag7603
• Full Name: peptidylprolyl isomerase E (cyclophilin E)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC004898
• Gene Symbol: Cyclophilin E
• Gene ID (NCBI): 10450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UNP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).