Tested Applications
Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, BxPC-3 cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85952-1-RR targets Cyclophilin E in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Cyclophilin E fusion protein Ag7603 Predict reactive species |
Full Name | peptidylprolyl isomerase E (cyclophilin E) |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC004898 |
Gene Symbol | Cyclophilin E |
Gene ID (NCBI) | 10450 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UNP9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).
Protocols
Product Specific Protocols | |
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WB protocol for Cyclophilin E antibody 85952-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |