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Cytokeratin 16 Monoclonal antibody
Cytokeratin 16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2H4D8
Cat no : 66802-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, human milk, A549 cells |
Positive IHC detected in | human tonsillitis tissue, human cervical cancer tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
Product Information
66802-1-Ig targets Cytokeratin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Cytokeratin 16 fusion protein Ag11240 |
Full Name | keratin 16 |
Calculated Molecular Weight | 473 aa, 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC039169 |
Gene Symbol | Cytokeratin 16 |
Gene ID (NCBI) | 3868 |
RRID | AB_2882145 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
IHC protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
IF protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
FC protocol for Cytokeratin 16 antibody 66802-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.
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Nat Commun Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer | ||
Genomics Identification of prognostic biomarkers for cholangiocarcinoma by combined analysis of molecular characteristics of clinical MVI subtypes and molecular subtypes
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