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Cytokeratin 16 Monoclonal antibody

Cytokeratin 16 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H4D8

Cat no : 66802-1-Ig

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman skin cancer tissue, human cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66802-1-Ig targets Cytokeratin 16 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag11240
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC039169
Gene symbol KRT16
Gene ID (NCBI) 3868
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 16 antibody 66802-1-IgDownload protocol
IHC protocol for Cytokeratin 16 antibody 66802-1-IgDownload protocol
IF protocol for Cytokeratin 16 antibody 66802-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cancers (Basel)

Emerging Insights into Keratin 16 Expression during Metastatic Progression of Breast Cancer.

Authors - Maha Elazezy
  • KD Validated