CoraLite® Plus 488-conjugated Cytokeratin 16 Monoclonal antibody

• 

  WB analysis using CL488-66802

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66802 (Cytokeratin 16 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

Cytokeratin 16 Monoclonal Antibody for WB

Cat No. CL488-66802

Clone No.2H4D8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB

Synonyms

KRT16, 2H4D8, CK 16, CK16, CK-16

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66802 targets Cytokeratin 16 in WB applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11240

  Product name: Recombinant human KRT16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 121-473 aa of BC039169

  Sequence: MQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

• Full Name: keratin 16
• Calculated Molecular Weight: 473 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC039169
• Gene Symbol: Cytokeratin 16
• Gene ID (NCBI): 3868
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08779
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.