Cytokeratin 19 Recombinant antibody, PBS Only

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  WB analysis using 86250-1-RR (same clone as 86250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 86250-1-RR (same clone as 86250-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 86250-1-RR (same clone as 86250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86250-1-RR (Cytokeratin 19 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86250-1-RR (same clone as 86250-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 19 antibody (86250-1-RR, Clone: 250894B1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86250-1-PBS in a different storage buffer formulation.

Cytokeratin 19 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86250-1-PBS

Clone No.250894B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CK 19, CK19, KRT19, CK-19, Cytokeratin,Keratin

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Product Information

86250-1-PBS targets Cytokeratin 19 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 19
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC002539
• Gene Symbol: Cytokeratin 19
• Gene ID (NCBI): 3880
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08727
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.