Cytokeratin 39 Recombinant monoclonal antibody, PBS Only

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• Cytokeratin 39 Recombinant monoclonal antibody, PBS Only (86277-2-PBS)

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  WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86277-2-RR (same clone as 86277-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86277-2-RR (KRT39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse esophagus using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 86277-2-RR (Cytokeratin 39 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse skin using 86277-2-RR (same clone as 86277-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 86277-2-RR (same clone as 86277-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cytokeratin 39 antibody (86277-2-RR, Clone: 250891F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86277-2-PBS in a different storage buffer formulation.

Cytokeratin 39 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 86277-2-PBS

Clone No.250891F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

CK-39, Cytokeratin-39, K39, KA35, Keratin, type I cytoskeletal 39

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Product Information

86277-2-PBS targets Cytokeratin 39 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 39
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC157887
• Gene Symbol: KRT39
• Gene ID (NCBI): 390792
• RRID: AB_3744775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6A163
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 39 (CK-39) is a member of the cytokeratin family, a group of intermediate filament proteins primarily expressed in epithelial cells. Cytokeratins are classified into Type I (acidic) and Type II (basic) subfamilies, which pair to form heteropolymers essential for maintaining cellular structural integrity, mechanical stability, and stress resistance. CK-39 is predominantly expressed in stratified and glandular epithelia, such as those found in the prostate, bladder, and respiratory tract. Its expression pattern may vary depending on tissue differentiation states.