Cytokeratin 5 Polyclonal antibody

Cytokeratin 5 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 28506-1-AP

Synonyms

58 kDa cytokeratin, Cell and organelle markers, CK 5, CK5, Cytokeratin 5, Cytoskeleton Marker, K5, keratin 5, KRT5, KRT5A, Type II keratin Kb5

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Validation Data Gallery

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  WB analysis using 28506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 28506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 28506-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 5 (28506-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissues, rat skin tissues
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, human tonsillitis tissue
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 3 publications below

Product Information

28506-1-AP targets Cytokeratin 5 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 5 fusion protein Ag25436
• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: KRT5
• Gene ID (NCBI): 3852
• RRID: AB_2881159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	J Invest Dermatol; Circulating Exosomal miR-493-3p Affects Melanocyte Survival and Function by Regulating Epidermal Dopamine Concentration in Segmental Vitiligo.; Authors - Li Dong
mouse	IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
human	WB	J Clin Lab Anal; Hsa_circRNA_0017620 regulated cell progression of non-small-cell lung cancer via miR-520a-5p/KRT5 axis.; Authors - Shan Chen
mouse	IF	J Oral Biosci; Epigenetic modifier G9a is involved in regulation of mouse tongue development; Authors - Hisashi Ideno