Cytokeratin 5 Polyclonal antibody

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• Cytokeratin 5 Polyclonal antibody (28506-1-AP)

• KD/KO Validated

Cytokeratin 5 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 28506-1-AP Copied!

Publications (17)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA
For FC, select conjugated version.

Synonyms

CK 5, CK-5, keratin 5, KRT5, 58 kDa cytokeratin

• 

  WB analysis of A431 using 28506-1-AP

  WB result of Cytokeratin 5 antibody (28506-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytokeratin 5 transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 28506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brown disease using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 28506-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28506-1-AP (Cytokeratin 5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human tonsillitis using 28506-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 28506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 28506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 5 antibody (28506-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissues, rat skin tissues
• Positive IHC detected in: human tonsillitis tissue, human brown disease, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28506-1-AP targets Cytokeratin 5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25436

  Product name: Recombinant human KRT5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 6-60 aa of BC024292

  Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY

• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• RRID: AB_2881159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13647
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells

Publications

What published studies show

The Cytokeratin 5 Polyclonal antibody (Cat# 28506-1-AP) has 17 citations published across 14 journals, including Nature Communications, Bioactive Materials, Cancer Letters, Small, International Journal of Biological Sciences, Stem Cell Research & Therapy, Journal of Investigative Dermatology, Journal of Materials Chemistry B, Human Gene Therapy, FASEB Journal, Journal of Clinical Laboratory Analysis, Journal of Oral Biosciences, Discovery Oncology, and Clinical and Experimental Otorhinolaryngology. Research fields span cancer immunotherapy, organoid biology, stem cell research, wound healing, dermatology, reproductive biology, and epigenetics.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Bioact Mater; Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture; Authors - Jiaxin Wang
human	IF	Bioact Mater; Probiotic-inspired hybrid nanovesicles for enhancing immune checkpoint therapy efficiency via tumor immune microenvironment modulation.; Authors - Fang Wang
human	IF	Nat Commun; An iPSC-based in vitro model recapitulates human thymic epithelial development and multi-lineage specification.; Authors - Yann Pretemer
		Nat Commun; Nanostructured organic sheets sequestering small extracellular vesicles and reactive species to protect against radiation-induced mucositis; Authors - Yuefei Zhu
mouse	WB	Small; An Engineered Cytokine-Induced Killer Cell-Derived Nanovesicle Featuring Multiplexed Immune Activation Performances for Enhanced Tumor Therapy.; Authors - Tong Wang
human	IF	Cancer Lett; The Single Cell Immunogenomic Landscape after Neoadjuvant Immunotherapy combined Chemotherapy in Esophageal Squamous Cell Carcinoma; Authors - Zhe Yi Wang