CoraLite®555-conjugated Cytokeratin 5 Polyclonal antibody

• 

  IF Staining of human tonsillitis using CL555-28506

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:200.

• 

  IF Staining of human tonsillitis using CL555-28506

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:200.

• 

  IF Staining of A431 using CL555-28506

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite®555 Cytokeratin 5 antibody (CL555-28506) at dilution of 1:400.

• 

  FC experiment of A431 using CL555-28506

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®555-conjugated Cytokeratin 5 Polyclonal antibody (CL555-28506)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Cytokeratin 5 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL555-28506

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

CK 5, CK-5, keratin 5, KRT5, 58 kDa cytokeratin

---

Tested Applications

• Positive IF-P detected in: human tonsillitis tissue
• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-28506 targets Cytokeratin 5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25436

  Product name: Recombinant human KRT5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 6-60 aa of BC024292

  Sequence: SVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGY

• Full Name: keratin 5
• Calculated Molecular Weight: 590 aa, 62 kDa
• GenBank Accession Number: BC024292
• Gene Symbol: Cytokeratin 5
• Gene ID (NCBI): 3852
• RRID: AB_2919650
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Excitation Laser: Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13647
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cytokeratin 5 (CK5) is a type II intermediate filament protein predominantly expressed in basal epithelial cells of various tissues, including the skin, breast, salivary glands, urinary tract, and upper aerodigestive tract. It is commonly found in specialized cell types such as squamous cells, basal cells, and myoepithelial cells. CK5 forms heterodimers with cytokeratin 14 and plays a crucial role in providing structural support to epithelial cells