Cytokeratin 6B Recombinant antibody

• 

  WB analysis using 85423-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85423-2-RR (KRT6B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85423-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85423-2-RR against Human Cytokeratin 6B were performed. The affinity constant is 4.41 nM.

Cytokeratin 6B Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85423-2-RR

Clone No.242874F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KRT6B, 242874F9, CK 6B, CK5/6, CK6B

---

Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85423-2-RR targets Cytokeratin 6B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 6B fusion protein Ag11288
• Full Name: keratin 6B
• Calculated Molecular Weight: 564 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC034535
• Gene Symbol: Cytokeratin 6B
• Gene ID (NCBI): 3854
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04259
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies.