Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2H1

Cat no : 68296-1-Ig

Synonyms

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7



Tested Applications

Positive WB detected inEC109 cells, HeLa cells, HaCat cells, A431 cells, A549 cells, BxPC-3 cells, T-47D cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human ovary tumor tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4150-1:16600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

68296-1-Ig targets Cytokeratin 7 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag7895
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
GenBank accession numberBC002700
Gene symbol KRT7
Gene ID (NCBI) 3855
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7 antibody 68296-1-IgDownload protocol
IHC protocol for Cytokeratin 7 antibody 68296-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols