Cytokeratin 7 Monoclonal antibody

Cytokeratin 7 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G2H1

Cat no : 68296-1-Ig

Synonyms

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Validation Data Gallery

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  WB analysis using 68296-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68296-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 68296-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68296-1-Ig (Cytokeratin 7 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: EC109 cells, HeLa cells, HaCat cells, A431 cells, A549 cells, BxPC-3 cells, T-47D cells
• Positive IHC detected in: human lung cancer tissue, human endometrial cancer tissue, human ovary tumor tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:4150-1:16600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68296-1-Ig targets Cytokeratin 7 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cytokeratin 7 fusion protein Ag7895
• Full Name: keratin 7
• Calculated Molecular Weight: 469 aa, 51 kDa
• GenBank Accession Number: BC002700
• Gene Symbol: KRT7
• Gene ID (NCBI): 3855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is a marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.