Cytokeratin 80 Recombinant monoclonal antibody

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  WB analysis using 86313-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86313-1-RR (Cytokeratin 80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skin using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 86313-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 80 antibody (86313-1-RR, Clone: 250883H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 80 Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 86313-1-RR

Clone No.250883H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

KRT80, CK 80, CK-80, Cytokeratin-80, K80

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NIH/3T3 cells
• Positive IF-P detected in: mouse skin tissue, human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86313-1-RR targets Cytokeratin 80 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 80
• Calculated Molecular Weight: 422 aa, 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC065180
• Gene Symbol: KRT80
• Gene ID (NCBI): 144501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6KB66
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRT80 is a human IF type II epithelial keratin gene involved in the formation of IF heterodimers in various epithelial cells. KRT80 is overexpressed in many neoplasms and plays an essential role in promoting cell proliferation, migration and invasiveness, and is associated with poor prognosis in cancer patients. KRT80 has three isoforms with predicted MW of 50 kDa, 47 kDa, and 54 kDa.