DAB2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 86141-1-RR (same clone as 86141-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 86141-1-RR (same clone as 86141-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  Sample test of MP02525-1

  The mean DAB2 concentration was determined to be 34.8 ng/mL in Hela cell extract based on a 1.1 mg/mL extract load.

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  IF Staining of RAW 264.7 using 86141-1-RR (same clone as 86141-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using DAB2 antibody (86141-1-RR, Clone: 250409B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86141-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02525-1

  Cytometric bead array standard curve of MP02525-1, DAB2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86141-1-PBS. Detection antibody: 86141-3-PBS. Standard: Ag0162. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86141-1-RR against Human DAB2 were performed. The affinity constant is 3.07 nM.

DAB2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86141-1-PBS

Clone No.250409B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2

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Product Information

86141-1-PBS targets DAB2 as part of a matched antibody pair:

MP02525-1: 86141-1-PBS capture and 86141-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DAB2 fusion protein Ag0162
• Full Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC003064
• Gene Symbol: DAB2
• Gene ID (NCBI): 1601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98082
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.