DAB2 Recombinant antibody

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  WB analysis using 86141-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 86141-1-RR

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 86141-1-RR (DAB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 86141-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed RAW 264.7 cells using DAB2 antibody (86141-1-RR, Clone: 250409B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86141-1-RR against Human DAB2 were performed. The affinity constant is 3.07 nM.

DAB2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86141-1-RR

Clone No.250409B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Adaptor molecule disabled-2, Differentially expressed in ovarian carcinoma 2, Differentially-expressed protein 2, Disabled homolog 2, DOC 2

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Tested Applications

• Positive WB detected in: HeLa cells, Caco-2 cells, A172 cells, RAW 264.7 cells
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86141-1-RR targets DAB2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DAB2 fusion protein Ag0162
• Full Name: disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC003064
• Gene Symbol: DAB2
• Gene ID (NCBI): 1601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P98082
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAB2 is a protein of 770 amino acid residues with a predicted molecular weight of 85.5kDa. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta). Human DAB2 has an overall 83% identify with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain (PID), and contains multiple SH3 binding motifs. Chromosomal localization by FISH showed that the DAB2 gene is located on 5p13. The expression of DAB2 is down-regulated or absent in all the carcinoma cell lines examined, including prostate and ovarian carcinoma cell lines. The N-terminal domain of DAB2 interacts with Dishevelled-3 (Dvl-3), a signaling mediator of the Wnt pathway. Ectopic expression of DAB2 attenuates canonical Wnt/catenin-mediated signaling, including accumulation of catenin and cyclin D1 induction. DAB2 suppresses both protein kinase C and peptide growth factor-elicited signal pathways via the Ras-mitogen-activated protein kinase pathway. The proline-rich domain of DAB2 also interacts with proteins containing SH3 domain, such as Src and Fgr. The binding of DAB2 to c-Src resultes in the inactivation of c-Src. All data suggest that DAB2 is a potent tumor suppressor in many cancer types.