DACH1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DACH1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B6D2

Cat no : 60082-1-PBS

Synonyms

DACH, DACH1, Dachshund homolog 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

  WB result of DACH1 (60082-1-Ig, 1:5000) with si-control and si-DACH1 transfected HEK293 cells. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60082-1-Ig (same clone as 60082-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60082-1-Ig (same clone as 60082-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 60082-1-Ig (same clone as 60082-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 60082-1-Ig (same clone as 60082-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60082-1-Ig (DACH1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60082-1-Ig (DACH1 antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60082-1-Ig (DACH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 60082-1-Ig (DACH1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse embryo using 60082-1-Ig (same clone as 60082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using DACH1 antibody (60082-1-Ig, Clone: 3B6D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60082-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60082-1-PBS targets DACH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DACH1 fusion protein Ag4474
• Full Name: dachshund homolog 1 (Drosophila)
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 97-110 kDa
• GenBank Accession Number: BC021219
• Gene Symbol: DACH1
• Gene ID (NCBI): 1602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.