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DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human, rat, mouse







Cat no : 60082-1-Ig


DACH, DACH1, Dachshund homolog 1

Tested Applications

Positive WB detected inMCF-7 cells, C6 cells, L02 cells, ROS1728 cells, HEK-293 cells
Positive IHC detected inmouse brain tissue, human endometrial cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, mouse brain tissue, mouse embryo tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60082-1-Ig targets DACH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DACH1 fusion protein Ag4474
Full Name dachshund homolog 1 (Drosophila)
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 97-110 kDa
GenBank Accession NumberBC021219
Gene Symbol DACH1
Gene ID (NCBI) 1602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.


Product Specific Protocols
WB protocol for DACH1 antibody 60082-1-IgDownload protocol
IHC protocol for DACH1 antibody 60082-1-IgDownload protocol
IF protocol for DACH1 antibody 60082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Biol Int

DACH1 inhibits the proliferation and migration of papillary thyroid carcinoma

Authors - Shengru Liang

Front Oncol

Identification and verification of the temozolomide resistance feature gene DACH1 in gliomas

Authors - Qiang Gu
  • KD Validated