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DACH1 Monoclonal antibody

DACH1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3B6D2

Cat no : 60082-1-Ig

Synonyms

DACH, DACH1, Dachshund homolog 1



Tested Applications

Positive WB detected inMCF-7 cells, C6 cells, L02 cells, ROS1728 cells, HEK-293 cells
Positive IHC detected inhuman endometrial cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, mouse brain tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60082-1-Ig targets DACH1 in WB, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DACH1 fusion protein Ag4474
Full Name dachshund homolog 1 (Drosophila)
Calculated molecular weight 79 kDa
Observed molecular weight 97-110 kDa
GenBank accession numberBC021219
Gene symbol DACH1
Gene ID (NCBI) 1602
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DACH1, a homologue of the Drosophila dachshund gene, is a key regulator of cell fate determination during eye, leg, and brain development in the fly. Through interacting with NCoR and Smad4, DACH1 is able to inhibit the transforming growth factor-beta (TGF-beta) signaling pathway. DACH1 can inhibit breast cancer cellular proliferation via cyclin D1, suggesting a possible role in tumor suppression. Additionally, DACH1 plays an important role in negative regulation of RANKL (Receptor activator of NF-kappaB ligand ) gene expression in marrow stromal/preosteoblast cells, and loss of DACH1 expression might be involved in endometrial cancer progression. Four isoforms of DACH1 are produced by alternative splicing, but isoform1(97-110kd) is the predominantly expressed form in tissue. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human DACH1.

Protocols

Product Specific Protocols
WB protocol for DACH1 antibody 60082-1-IgDownload protocol
IHC protocol for DACH1 antibody 60082-1-IgDownload protocol
IF protocol for DACH1 antibody 60082-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols