DAPK2-Specific Polyclonal antibody, PBS Only

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• DAPK2-Specific Polyclonal antibody, PBS Only (20048-1-PBS)

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  WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

  WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20048-1-AP (same clone as 20048-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 20048-1-AP (same clone as 20048-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAPK2-Specific antibody (20048-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DAPK2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20048-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

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Product Information

20048-1-PBS targets DAPK2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: death-associated protein kinase 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38-43 kDa
• GenBank Accession Number: NM_014326
• Gene Symbol: DAPK2
• Gene ID (NCBI): 23604
• RRID: AB_10667409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UIK4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.