DARS Monoclonal antibody

DARS Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C5D3

Cat no : 67930-1-Ig

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67930-1-Ig targets DARS in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DARS fusion protein Ag7059
Full Name aspartyl-tRNA synthetase
Calculated Molecular Weight 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC000629
Gene Symbol DARS
Gene ID (NCBI) 1615
RRIDAB_2918682
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DARS antibody 67930-1-IgDownload protocol
IHC protocol for DARS antibody 67930-1-IgDownload protocol
IF protocol for DARS antibody 67930-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols