DARS Monoclonal antibody

DARS Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C5D3

Cat no : 67930-1-Ig

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

---

Validation Data Gallery

• 

  WB analysis using 67930-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Product Information

67930-1-Ig targets DARS in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DARS fusion protein Ag7059
• Full Name: aspartyl-tRNA synthetase
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 50-57 kDa
• GenBank accession number: BC000629
• Gene symbol: DARS
• Gene ID (NCBI): 1615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.