DARS Monoclonal antibody, PBS Only

• 

  WB analysis using 67930-1-Ig (same clone as 67930-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67930-1-Ig (DARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 67930-1-Ig (same clone as 67930-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67930-1-Ig (DARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67930-1-Ig (same clone as 67930-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DARS antibody (67930-1-Ig, Clone: 3C5D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67930-1-PBS in a different storage buffer formulation.

DARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67930-1-PBS

Clone No.3C5D3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Aspartate tRNA ligase, aspartyl tRNA synthetase, AspRS, DARS, DKFZp781B11202

---

Product Information

67930-1-PBS targets DARS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7059

  Product name: Recombinant human DARS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 155-501 aa of BC000629

  Sequence: QLDDAVRPEAEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFSIGPVFRAEDSNTHRHLTEFVGLDIEMAFNYHYHEVMEEIADTMVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALAMLREAGVEMGDEDDLSTPNEKLLGHLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP

• Full Name: aspartyl-tRNA synthetase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC000629
• Gene Symbol: DARS
• Gene ID (NCBI): 1615
• RRID: AB_2918682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14868
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.