CCAR2 Polyclonal antibody

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  WB analysis using 22638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22638-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22638-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22638-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22638-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22638-1-AP (CCAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22638-1-AP

  IP result of anti-CCAR2 (IP:22638-1-AP, 4ug; Detection:22638-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22638-1-AP (CCAR2 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human breast cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:400.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IHC staining of human pancreas cancer using 22638-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer slide using 22638-1-AP (CCAR2 antibody at dilution of 1:50.

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  IF Staining of HepG2 using 22638-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA1967 antibody 22638-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CCAR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22638-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:8000-1:32000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

22638-1-AP targets CCAR2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18374

  Product name: Recombinant human DBC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 571-923 aa of BC018269

  Sequence: EPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN

• Full Name: KIAA1967
• Calculated Molecular Weight: 923 aa, 103 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC018269
• Gene Symbol: CCAR2
• Gene ID (NCBI): 57805
• RRID: AB_2879138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N163
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	CoIP,RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
mouse	WB, IF	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human	WB,IHC,IP	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu
mouse	IF	Br J Pharmacol; AdipoRon promotes amyloid-β clearance through enhancing autophagy via nuclear GAPDH-induced sirtuin 1 activation in Alzheimer's disease; Authors - Fengjiao Sun
human	WB	Redox Biol; SIRT1 activated by AROS sensitizes glioma cells to ferroptosis via induction of NAD+ depletion-dependent activation of ATF3; Authors - Xi Chen
rat	WB	Free Radic Biol Med; CircMETTL9 targets CCAR2 to induce neuronal oxidative stress and apoptosis via mitochondria-mediated pathways following traumatic brain injury; Authors - Hao Huang