CCAR2 Polyclonal antibody

CCAR2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22638-1-AP

Synonyms

ccar2, DBC.1, DBC-1, Deleted in breast cancer gene 1 protein, KIAA1967, NET35, p30 DBC, Protein KIAA1967



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, MCF-7 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22638-1-AP is CCAR2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374
Full Name KIAA1967
Calculated molecular weight 923 aa, 103 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC018269
Gene symbol KIAA1967
Gene ID (NCBI) 57805
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CCAR2 antibody 22638-1-APDownload protocol
IHC protocol for CCAR2 antibody 22638-1-APDownload protocol
IF protocol for CCAR2 antibody 22638-1-APDownload protocol
IP protocol for CCAR2 antibody 22638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols