DBH Polyclonal antibody

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  WB analysis using 10777-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10777-1-AP (DBH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10777-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10777-1-AP (DBH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 10777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10777-1-AP (DBH antibody at dilution of 1:100 (under 40x lens).

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  IF Staining of rat brain using 10777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using DBH antibody (10777-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 10777-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DBH antibody (10777-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 10777-1-AP

  Immunofluorescent analysis of HeLa cells using 10777-1-AP (Dopamine beta Hydroxylase antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 10777-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DBH (10777-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DBH Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Cat No. 10777-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Synonyms

DBM, Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, EC:1.14.17.1, Soluble dopamine beta-hydroxylase

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: rat brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells, HeLa cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

10777-1-AP targets DBH in WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1196

  Product name: Recombinant human DBH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 269-603 aa of BC017174

  Sequence: CDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG

• Full Name: DBH
• Calculated Molecular Weight: 64.8 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC017174
• Gene Symbol: DBH
• Gene ID (NCBI): 1621
• RRID: AB_2277037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09172
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DBH belongs to the copper type II ascorbate-dependent monooxygenase family and catalyzes the oxidative hydroxylation of DBH to norepinephrine. It is almost exclusively located in the adrenal medulla and the synaptic vesicles of postganglionic sympathetic neurons(PMID:11857564).The DBH is glycosylated (63--67 kDa) while still in the membrane(PMID:2325165) and it can form a dimer(PMID:8546710).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Int J Mol Sci; Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.; Authors - Shin Takasawa
human	WB	Peptides; Markers of the sympathetic, parasympathetic and sensory nervous system are altered in the human diabetic choroid.; Authors - Yuan-Jun Qin
mouse	IF	Tissue Cell; Autonomic nervous system imbalance in diabetic mouse choroids; Authors - Yuan-Jun Qin