CoraLite®594 Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody

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• CoraLite®594 Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (CL594-98205)

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  FC experiment of Transfected HEK-293T using CL594-98205

  1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 5 ul CoraLite®594 Anti-Human DC-SIGNR/CD299 Rabbit RecAb (CL594-98205, Clone:241683G8). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using CL594-98205

  1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 5 ul CoraLite®594 Anti-Human DC-SIGNR/CD299 Rabbit RecAb (CL594-98205, Clone:241683G8) (red) or CoraLite®594 Rabbit IgG Isotype Control Recombinant Antibody (CL594-98136, Clone: 240953C9) (blue). Cells were not fixed.

DC-SIGNR/CD299 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL594-98205

Clone No.241683G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CLEC4M, 241683G8, CD209 antigen like protein 1, CD209 antigen-like protein 1, CD209L

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-98205 targets DC-SIGNR/CD299 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: C-type lectin domain family 4, member M
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC038851
• Gene Symbol: DC-SIGNR/CD299
• Gene ID (NCBI): 10332
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H2X3
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN/CD209) and DC-SIGN related (DC-SIGNR/CD299/L-SIGN/CD209L/CLEC4M) are trans-membrane C-type lectins that are calcium-dependent carbohydrate-binding proteins, which can act as cell-adhesion and pathogen-recognition receptors (PMID: 18458800). DC-SIGN and DC-SIGNR share 77% amino acid identity (PMID: 10975799; 11226297). DC-SIGN is expressed on subsets of dendritic cells and macrophages while DC-SIGNR is restricted to endothelial cells of the liver, lymph nodes and placenta (PMID: 17509452). DC-SIGNR servers as a receptor for ICAM-3. It recognizes a wide range of evolutionarily divergent pathogens.