FcZero-rAb® PE Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody

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• FcZero-rAb® PE Anti-Human DC-SIGNR/CD299 Rabbit Recombinant Antibody (PE-FcA98205)

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  FC experiment of Transfected HEK-293T using PE-FcA98205

  1x10^6 HEK-293T cells or DC-SIGNR/CD299 transfected HEK-293T cells were surface stained with 5 ul PE Anti-Human DC-SIGNR/CD299 Rabbit RecAb (PE-FcA98205, Clone: 241683G8). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

DC-SIGNR/CD299 FcZero-rAb^® Recombinant Antibody for FC

Cat No. PE-FcA98205

Clone No.241683G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CLEC4M, CD209 antigen like protein 1, CD209 antigen-like protein 1, CD209L, CD209L1

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98205 targets DC-SIGNR/CD299 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: C-type lectin domain family 4, member M
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC038851
• Gene Symbol: DC-SIGNR/CD299
• Gene ID (NCBI): 10332
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H2X3
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Dendritic cell-specific intracellular adhesion molecule-3-grabbing non-integrin (DC-SIGN/CD209) and DC-SIGN related (DC-SIGNR/CD299/L-SIGN/CD209L/CLEC4M) are trans-membrane C-type lectins that are calcium-dependent carbohydrate-binding proteins, which can act as cell-adhesion and pathogen-recognition receptors (PMID: 18458800). DC-SIGN and DC-SIGNR share 77% amino acid identity (PMID: 10975799; 11226297). DC-SIGN is expressed on subsets of dendritic cells and macrophages while DC-SIGNR is restricted to endothelial cells of the liver, lymph nodes and placenta (PMID: 17509452). DC-SIGNR servers as a receptor for ICAM-3. It recognizes a wide range of evolutionarily divergent pathogens.