DCBLD2 Recombinant antibody, PBS Only

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  WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

  IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 85000-4-RR (same clone as 85000-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 85000-4-RR (same clone as 85000-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85000-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

DCBLD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 85000-4-PBS

Clone No.242604E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

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Product Information

85000-4-PBS targets DCBLD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCBLD2 fusion protein Ag32823
• Full Name: discoidin, CUB and LCCL domain containing 2
• Calculated Molecular Weight: 775 aa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC029658
• Gene Symbol: DCBLD2
• Gene ID (NCBI): 131566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96PD2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).