DCBLD2 Recombinant antibody

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  WB analysis using 85000-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 85000-4-RR

  IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug.

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  IHC staining of human colon cancer using 85000-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85000-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 85000-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 85000-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85000-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

DCBLD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 85000-4-RR

Clone No.242604E8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

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Tested Applications

• Positive WB detected in: A549 cells, U2OS cells
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human colon cancer tissue, human rectal cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85000-4-RR targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCBLD2 fusion protein Ag32823
• Full Name: discoidin, CUB and LCCL domain containing 2
• Calculated Molecular Weight: 775 aa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC029658
• Gene Symbol: DCBLD2
• Gene ID (NCBI): 131566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96PD2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).