DCC Recombinant antibody

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  WB analysis of A-673 using 85787-4-RR

  A-673 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U266 using 85787-4-RR

  U266 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

DCC Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85787-4-RR

Clone No.250085A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Colorectal cancer suppressor, CRC18, CRCR1, IGDCC1

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Tested Applications

• Positive WB detected in: A-673 cells, U266 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85787-4-RR targets DCC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCC fusion protein Eg2937
• Full Name: deleted in colorectal carcinoma
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_005215.3
• Gene Symbol: DCC
• Gene ID (NCBI): 1630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43146
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).