DCC Recombinant antibody, PBS Only (Capture)

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  WB analysis of A-673 using 85787-4-RR (same clone as 85787-4-PBS)

  A-673 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

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  WB analysis of U266 using 85787-4-RR (same clone as 85787-4-PBS)

  U266 cells were subjected to SDS PAGE followed by western blot with 85787-4-RR (DCC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85787-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02122-3

  Sandwich ELISA standard curve of MP02122-3, Human DCC Recombinant Matched Antibody Pair - PBS only. 85787-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2937. 85787-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP02122-3

  The mean DCC concentration was determined to be 11.21 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load and 210.63 ng/mL in mouse brain tissue extract based on a 6.60 mg/mL extract load.

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  Cytometric bead array standard curve of MP02122-1

  Cytometric bead array standard curve of MP02122-1, DCC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85787-4-PBS. Detection antibody: 85787-3-PBS. Standard: Eg2937. Range: 0.781-100 ng/mL.

DCC Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85787-4-PBS

Clone No.250085A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Colorectal cancer suppressor, CRC18, CRCR1, IGDCC1

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Product Information

85787-4-PBS targets DCC as part of a matched antibody pair:

MP02122-1: 85787-4-PBS capture and 85787-3-PBS detection (validated in Cytometric bead array)

MP02122-3: 85787-4-PBS capture and 85787-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCC fusion protein Eg2937
• Full Name: deleted in colorectal carcinoma
• Calculated Molecular Weight: 158 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_005215.3
• Gene Symbol: DCC
• Gene ID (NCBI): 1630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43146
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody raised against a synthesized polypeptide of human DCC protein recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).