Tested Applications
| Positive WB detected in | COLO 320 cells, BxPC-3 cells, HEK-293 cells, human brain tissue, human kidney tissue, human lung tissue, MOVAS cells, HeLa cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11535-1-AP targets DCI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2094 Product name: Recombinant human DCI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC020228 Sequence: MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG Predict reactive species |
| Full Name | dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase) |
| Calculated Molecular Weight | 302 aa, 33 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC020228 |
| Gene Symbol | DCI |
| Gene ID (NCBI) | 1632 |
| RRID | AB_2089906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DCI antibody 11535-1-AP | Download protocol |
| IHC protocol for DCI antibody 11535-1-AP | Download protocol |
| WB protocol for DCI antibody 11535-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DCI Polyclonal antibody (catalog number 11535-1-AP) has 3 total citations. These appear in Cell, Developmental Cell, and Biomolecules. The associated research fields include metastatic extravasation and colonization (ACSL4 and polyunsaturated lipids), ferroptosis driven by mitochondrial CCN1 via fatty acid β-oxidation, and exercise-induced cardioprotection mediated by the FTO-Eci1 axis in pressure overload mice. All citations used the antibody for Western blot (WB) applications in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Biomolecules FTO-Eci1 Axis Mediates Exercise-Induced Cardioprotection in Pressure Overload Mice.
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